Idayi yeReticulocyte yeSysmex Hematology Analyzers

Idayi ye-Reticulocyte ye-Sysmex Hematology Analyzers ibonelela ngokuchongwa kwe-nucleic acid ye-fluorescent kwi-semiconductor laser flow cytometry platforms. I-reagent ye-liquid ingena kwiiseli zegazi ze-nucleated, i-reticulocytes, kunye neeplatelet ukuze zibophe ngokukhethiweyo nge-intracellular RNA kunye ne-DNA, ivelisa i-fluorescence esecaleni kunye neempawu zokusasaza ukukhanya kwiindlela ezichongiweyo zokufumanisa. Yenzelwe ukudityaniswa ngokuthe ngqo kunye neenkqubo ezizenzekelayo ze-fluorescence, isisombululo sigcina ubunzulu be-fluorescence obuzinzile, ingxolo ephantsi yangasemva, kunye neqashiso lokuvelisa kwakhona{2}}ukuze{3}}kusebenze kakhulu kwiisethingi zelebhu yeklinikhi.

I-automated hematology reagents zedayi ze-fluorescent zolwelo iziphawuli ezenzelwe ukuqukuqela-cytometry{1}ngokusekwe kumaqonga ohlalutyo lweeseli. Ngokungafaniyo namabala esmear enziwe ngesandla segazi asetyenziswa kwi-microscopy ekhanyayo, ezi dayi zolwelo zingena kwiiseli ekujoliswe kuzo ngokulawulwa kwe-membrane permeabilization ukulebhelisha i-intracellular nucleic acids. Ngaphantsi kwe-semiconductor laser excitation ngaphakathi kwe-optical flow cell, izakhiwo zeselula ezibhaliweyo zikhupha i-fluorescence esecaleni kunye neempawu zokusasaza ukukhanya, ukuvumela i-optics yenkqubo ukuba yenze i-scattergrams ezimbini ezichanekileyo kunye ne-cell cluster distributions.

Kuhlalutyo lwe-hematology ye-Optical hematology, iidayi ze-fluorescent zenza ucalucalulo lweeseli ezahlukeneyo ngokulinganisa intracellular nucleic acid density:

Umahluko kwiSeli yeGazi eMhlophe:Ukwahlula i-lymphocytes, i-monocytes, i-neutrophils, i-eosinophils, kunye ne-basophils ngokusekelwe kwi-RNA / DNA ratios kunye nesakhiwo se-cytoplasmic.

Ukuchongwa kweSeli engaqhelekanga kunye neNgakhulanga:Ichonga iiseli eziqhumayo kunye neegranulocyte ezingekakhuli ngokunyuka kwe-fluorescence ephumayo.

Ubalo lweReticulocyte:Ilinganisa intsalela ye-ribosomal RNA kwiiseli ezincinci zegazi ezibomvu zokuvavanya umongo wethambo umsebenzi we-erythropoietic.

Ukubala kwePlatelet yokubona:Isombulula iiplatelet ezinkulu kwaye yahlule amaqhekeza eeseli ezibomvu zegazi kwiiplatelet kusetyenziswa i-nucleic acid fluorescence gating ekujoliswe kuyo.

Idayi yefluorescent isebenza kwiitshaneli ezithile ezizihambelayo zamehlo:

Ijelo le-WNR:Ibala iiseli ezimhlophe zegazi kwaye ihlukanise iiseli zegazi ezibomvu ze-nucleated ngokusebenzisa i-nucleic acid staining.

Ijelo le-WDF:Ukwahlula i-leukocyte subpopulations evuthiweyo kwaye ifumanisa i-granulocyte engekavuthwa isebenzisa i-fluorescence yecala kunye neeparamitha zokuchitha icala.

Ijelo leWPC:Izikrini zeeseli ze-progenitor kunye ne-lymphocytes engaqhelekanga ngokusekelwe kubuninzi benyukliya kunye nokwahlukana kwe-membrane.

Ijelo leRET:Ilinganisa amaqhezu e-reticulocyte kunye neeparamitha zeeseli ezibomvu zegazi ngokusebenzisa i-RNA fluorescence intensity.

Ijelo le-PLT:Ilebhile iiplatelet nucleic acids ukuphelisa ukuphazamiseka kweeseli ezibomvu zegazi nge-microcytic ngexesha lokubala kwamehlo.

Yenzelwe ulungelelwaniso lokusebenza ngokuthe ngqo kunye namaqonga e-hematology azenzekelayo asebenzisa i-semiconductor laser flow cytometry:

XN Series:XN-1000, XN-2000, XN-3000, XN-9000, XN-L Series (XN-350, XN-450, XN-550)

XS Series:XS-500i, XS-800i, XS-1000i

XT Series:XT-1800i, XT-2000i, XT-4000i

XE Series:XE-2100, XE-5000

I-WNR yeSitishi Staining (STAC{0}}DYE{1}}SYSWNR):I-Permeabilizes cell membranes ukuvumela ukungena kwedayi yenyukliya ngokukhawuleza, ukwahlula i-NRBC nuclei kwiiseli ezimhlophe zegazi ukwenzela i-NRBC % kunye ne-WBC count echanekileyo.

I-WDF Channel Staining (STAC{0}}DYE{1}}SYSWDF):Amabala e-intracellular nucleic acids ye-leukocytes ukuvelisa amaqela ahlukeneyo e-scattergram ye-neutrophils, i-lymphocytes, i-monocytes, kunye ne-eosinophils.

I-WPC Channel Staining (STAC{0}}DYE{1}}SYSWPC):Ngokukhethekileyo amabala aqhuma iiseli kunye ne-lymphocyte engaqhelekanga esekwe kukwakheka kwelipid kunye noxinzelelo lwenyukliya kwiflegi yesibini yeeseli.

I-RET yeSitishi Staining (STAC{0}}DYE{1}}SYSRET):Ibophelela kwi-RNA eshiyekileyo kwiiseli zegazi ezibomvu ezingekavuthwa, ivumela ukubalwa kwepesenti ye-reticulocyte iyonke, ukubala okupheleleyo, kunye neqhezu le-reticulocyte elingakhulanga.

I-PLT yeSitishi Staining (STAC{0}}DYE{1}}SYSPLT):Ilebhile ii-nucleic acid zeplatelet ukunqanda uphazamiseko lwe-microcytic yeseli ebomvu yegazi ngexesha elisezantsi{0}}lomlinganiselo weplatelet optical optical.

Ulungelelwaniso lokuPakisha:

• Iikhasethi zekhatriji: 2 × 12 mL
• Isisombululo sebhotile: 1 × 50 mL, 1 × 100 mL, 1 × 500 mL

Ubushushu boGcino:I-2℃ukuya kwi-30℃(Khusela ekukhanyeni okuthe ngqo kunye nokubanda)
Ubomi beShelufu obungavulwanga:Iinyanga ezili-18
Kwi-Uzinzo lwebhodi:Iintsuku ezingama-60 kwiimeko zaselabhoratri ezingqongileyo

Izisombululo zeHematology Diluent:Izisombululo ze-Isotonic buffer ezigcina ingqibelelo yevolumu yeseli kunye nokulawula ukuhanjiswa kwamanzi ngexesha lemilinganiselo yomzimba.
IiReagents zeLyse zoMahluko weLeukocyte:Izisombululo ezisebenzayo ezikhetha iiseli ezibomvu zegazi ukulungiselela iiseli ezimhlophe zegazi kuhlalutyo lwamehlo.
Izisombululo zokuCoca isiHlalutyi:Ulwelo lokuhlamba lwe-Enzymatic oluyilelwe ukucacisa iprotein eyakhiwe kunye nentsalela yedayi evela kwimigca engaphakathi kunye neeseli ezijikelezayo.
Ulwelo olugxininisiweyo lweSheath:Ulwelo lwe-Hydrodynamic oluyilwe ukuba lube sembindini wesampulu yemijelo ngaphakathi kumagumbi okubona amehlo.

Iifayile ezishisayo: Idayi ye-reticulocyte ye-sysmex hematology analyzers, idayi ye-reticulocyte yaseTshayina ye-sysmex hematology analyzers abavelisi, ababoneleli